DEVELOPMENT, SECURITY, AND COOPERATION

Biomedical Lab Training Course for Scientists from Central/Eastern Europe and the Newly Independent States

Schedule for the first week of the course (3-10 June)

Sunday, 3 June 2001

All day Check-in at the Hotel of the Institute of Biochemistry and Biophysics (IBB), ul. Pawinskiego, 5f. Information packets including badges, city maps, and textbooks (for those who did not receive them by mail) will be left at the reception desk. Since everyone is arriving on different schedules, all day today is free for individual sightseeing or rest.

18:00 Kelly Robbins will be at the IBB Hotel to distribute per diem and travel reimbursement payments (she will also be available after dinner for those who cannot be there before)

19:00 Dinner in the IBB cafeteria

Monday, 4 June 2001

8:00 - 9:00 Breakfast is served at the hotel

9:00 - 10:30 General Introduction to Course (Dr. R. Andrew Byrd) - IBB Room 7 (Hall A, ground floor)

10:30 - 12:30 Lab Session I

12:30 - 13:30 Lunch in the IBB Cafeteria

13:30 - 14:30 Lecture: Multidimensional Spectroscopy (Dr. R. Andrew Byrd) - IBB Room 7

14:30 - 17:00 Lab Session II

17:15 - 18:00 Public Lecture (Topic and Speaker to be Determined) - IBB room E (Hall B, 1st floor)

18:00 - 20:00 Welcome Reception and Dinner - IBB Cafeteria

20:00 - 21:00 Tutorial and Discussion Session - IBB Room 7

Tuesday, 5 June 2001

8:00 - 9:00 Breakfast is served at the hotel

9:00 - 10:00 Data Analysis (Dr. Kevin Gardner) - IBB Room 7

10:00 - 12:30 Lab Session I

12:30 - 13:30 Lunch in the IBB Cafeteria

13:30 - 14:30 Lecture: Isotopic Labeling (Dr. Kevin Gardner) - IBB Room 7

14:30 - 17:00 Lab Session II

17:15 - 18:00 Public Lecture (Topic and Speaker to be Determined) - IBB Room E

18:00 - 20:00 Dinner in the IBB Cafeteria

20:00 - 21:00 Tutorial and Discussion Session - IBB Room 7

Wednesday, 6 June 2001

8:00 - 9:00 Breakfast is served at the hotel

9:00 - 10:00 Dipolar Coupling I (Dr. Eike Brunner) - IBB Room 7

10:00 - 12:30 Lab Session I

12:30 - 13:30 Lunch in the IBB Cafeteria

13:30 - 14:30 Lecture: Structural Calculation I (Dr. Michael Nilges) - IBB Room 7

14:30 - 17:00 Lab Session II

17:15 - 18:00 Public Lecture (Topic and Speaker to be Determined) - IBB Room E

18:00 - 20:00 Dinner in the IBB Cafeteria

20:00 - 21:00 Tutorial and Discussion Session - IBB Room 7

Thursday, 7 June 2001

8:00 - 9:00 Breakfast is served at the hotel

9:00 - 10:00 Structural Calculation II (Dr. Michael Nilges) - IBB Room 7

10:00 - 12:30 Lab Session I

12:30 - 13:30 Lunch in the IBB Cafeteria

13:30 - 14:30 Lecture: Dipolar Coupling II (Dr. Eike Brunner) - IBB Room 7

14:30 - 17:00 Lab Session II

17:15 - 18:00 Public Lecture (Topic and Speaker to be Determined) - IBB Room E

18:00 - 20:00 Dinner in the IBB Cafeteria

20:00 - 21:00 Tutorial and Discussion Session - IBB Room 7

Friday, 8 June 2001

8:00 - 9:00 Breakfast is served at the hotel

9:00 - 10:00 Structure Analysis (Dr. Walter Chazin) - IBB Room 7

10:00 - 12:30 Lab Session I

12:30 - 13:30 Lunch in the IBB Cafeteria

13:30 - 14:30 Lecture: Dynamics (Dr. Andrzej Ejchart) - IBB Room 7

14:30 - 17:00 Lab Session II

17:15 - 18:00 Public Lecture (Topic and Speaker to be Determined) - IBB Room E

18:00 - 20:00 Dinner in the IBB Cafeteria

20:00 - 21:00 Tutorial and Discussion Session - IBB Room 7

Saturday, 9 June 2001

8:00 - 9:00 Breakfast is served at the hotel

9:00 - 10:00 Lecture: Coupling Structure and Dynamics to Biology - IBB Room 7

10:00 - 12:00 Group Presentations, Roundtable Discussion, Evaluation - IBB Room 7

12:00 - 13:00 Lunch in the IBB Cafeteria

13:00 - 16:00 Free time for packing, rest, individual sightseeing in local area

16:00 Departure from IBB to Warsaw Old Town for sightseeing and dinner

Sunday, 10 June 2001

8:00 - 9:00 Breakfast served at hotel

10:00 Departure for Poznan by bus (5-hour trip - we will stop on the way for lunch)

16:00 Arrival in Poznan, accommodation at the conference center of the Institute of Bioorganic Chemistry (IBCH)

19:00 Dinner in the IBCH cafeteria

Schedule for the second week of the course (11-17 June)

Monday, 11 June 2001

8:00 - 9:00 Breakfast is served at the hotel

9:00 - 11:30 Lab Session I

11:30 - 12:30 Lecture: Introduction to Protein Crystallography (Dr. Lennart Sjolin)

12:30 - 13:30 Lunch in the IBCH Cafeteria

13:30 - 14:30 Lecture: Crystallization of Proteins (Dr. Alexander McPherson)

14:30 - 17:00 Lab Session II

17:15 - 18:00 Public Lecture: Structure of a Novel Serine-Carboxyl Proteinase (Dr. Alexander Wlodawer)

18:15 - 20:00 Dinner and Rest

20:00 - 21:00 Tutorial and Discussion Session

Tuesday, 12 June 2001

8:00 - 9:00 Breakfast is served at the hotel

9:00 - 11:30 Lab Session I

11:30 - 12:30 Lecture: Theory of Diffraction (Dr. Mariusz Jaskólski)

12:30 - 13:30 Lunch in the IBCH Cafeteria

13:30 - 14:30 Lecture: Crystallographic Data Collection (Dr. Zbigniew Dauter)

14:30 - 17:00 Lab Session II

17:15 - 18:00 Public Lecture: Can the Structure of a Bacterial Enzyme Explain Proteasome Activation? (Dr. Matthias Bochtler)

18:15 - 20:00 Dinner and Rest

20:00 - 21:00 Tutorial and Discussion Session

Wednesday, 13 June 2001

8:00 - 9:00 Breakfast is served at the hotel

9:00 - 11:30 Lab Session I

11:30 - 12:30 Lecture: Structure Solution (MIR/SIR) (Dr. Lennart Sjolin)

12:30 - 13:30 Lunch in the IBCH Cafeteria

13:30 - 14:30 Lecture: Structure Solution (MAD) (Dr. Matthias Bochtler)

14:30 - 17:00 Lab Session II

17:15 - 18:00 Public Lecture: Anomalous Scattering and High Resolution Structures (Dr. Zbigniew Dauter)

18:15 - 20:00 Dinner and Rest

20:00 - 21:00 Tutorial and Discussion Session

Thursday, 14 June 2001

8:00 - 9:00 Breakfast is served at the hotel

9:00 - 11:30 Lab Session I

11:30 - 12:30 Lecture: Structure Solution (MR) (Dr. Matthias Bochtler)

12:30 - 13:30 Lunch in the IBCH Cafeteria

13:30 - 14:30 Lecture: Model Building (Dr. Grzegorz Bujacz)

14:30 - 17:00 Lab Session II

17:15 - 18:00 Public Lecture: Electron Transfer Mechanism (Dr. Lennart Sjolin)

18:15 - 20:00 Dinner and Rest

20:00 - 21:00 Tutorial and Discussion Session

Friday, 15 June 2001

8:00 - 9:00 Breakfast is served at the hotel

9:00 - 11:30 Lab Session I

11:30 - 12:30 Lecture: Refinement, Part I (Dr. Alexander Wlodawer)

12:30 - 13:30 Lunch in the IBCH Cafeteria

13:30 - 14:30 Lecture: Refinement, Part II (Dr. Lennart Sjolin)

14:30 - 17:00 Lab Session II

17:15 - 18:00 Public Lecture: Applications of Atomic Force Microscopy to Macromolecular Crystallography (Dr. Alexander McPherson)

18:15 - 20:00 Dinner and Rest

20:00 - 21:00 Tutorial and Discussion Session

Saturday, 16 June 2001

8:00 - 9:00 Breakfast is served at the hotel

9:00 - 10:00 Validation/Comparison with NMR (Dr. Zbigniew Dauter)

10:00 - 11:00 Final Quality Checks and Deposition (Dr. Mariusz Jaskólski)

11:00 - 12:00 Summary of the Course (Dr. Alexander Wlodawer)

12:00 - 13:00 Lunch in the IBCH Cafeteria

13:00 Departure for excursion to local castle and other sites, followed by dinner in a country restaurant

Sunday, 17 June 2001

8:00 - 9:00 Breakfast is served at the hotel (for those who do not have to depart earlier in the morning)

Departure of instructors and students for home!

Schedule of Laboratory Practicals for IBB/Warsaw

All lab sessions will be held in the IBB NMR Labs (Rooms 35-38, Hall B, Floor -1)

There will be 5 groups of 5 - A, B, C, D, and E

Day	NMR I AM	NMR II PM	Data Proc. AM	Data Assn/Anal. PM	StrCalc. I AM	StrCalc. II PM	StrAnal. I AM	StrAnal. II PM	Dipolar Coupling AM	Dynamics PM
	Byrd	Byrd	Gardner	Gardner	Nilges	Nilges	Chazin	Chazin	Brunner	Ejchart
MON	A	A	B+E	B			C+D	C+D		E
TUE	C	C	D	D+E	A+B	A+B			E
WED	B	B			C+D	C+D	E	E	A	A
THU	D	D	C	C	E	E	A	A	B	B
FRI	E	E	A	A			B	B	C+D	C+D

Schedule of Laboratory Practicals for IBCH/Poznan

Students will be divided into 5 groups (5 students per group).

Each day Monday-Friday, the schedule will be the same, with groups rotating.

PM Structure solution Zbigniew Dauter & Matthias Bochtler

PM Crystallization A. McPherson & Grzegorz Bujacz

PM Structure solution (MR) Mariusz Jaskolski

PM Structure refinement Lennart Sjolin

PM Validation and deposition Alexander Wlodawer